Signalling
PelB

Part:BBa_K4223000:Design

Designed by: RongKai Tang   Group: iGEM22_HainanU_China   (2022-09-18)


pelB signal sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pelB signal sequence pelB is a secretory signal peptide that is widely used to export foreign proteins into the E. coli cytoplasmic space or secrete them directly into the extracellular medium. When attached to one protein, the protein is directed to the periplasmic membrane of E. coli, where the sequence is removed by the pelB peptidase. It is used to encapsulate protein antigen fusions directly onto the cell surface.



Source

References